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Cell Signaling Technology Inc anti sting
Anti Sting, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 1321 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sting+13647s/STING+Rabbit+mAb/pmc13011919-263-19-21
Average 98 stars, based on 1321 article reviews

anti sting - by Bioz Stars, 2026-09

98/100 stars

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Binding Assay:

Article Title: Cyclic-di-GMP induces inflammation and acute lung injury through direct binding to MD2.
Article Snippet: Recombinant human MD2 (rhMD2; 1787-MD-050/CF) protein was obtained from R&D Systems. .. Antibodies against p38 (9212S), phosphorylated (p-) p38 (Thr180/Tyr182, 9211S), c-Jun N-terminal kinase (JNK, 9252S), phosphorylated (p-) JNK (Thr183/Tyr185, 4668S), extracellular signal-regulated kinase (ERK, 4695S), phosphorylated (p-) ERK (Thr202/Tyr204, 4370S), glyceraldehyde-3-phosphate dehydrogenase 1 (GAPDH, 5174S), TANK binding protein 1 (TBK1, 38066S), phosphorylated (p-) TBK1(Ser172, 5483S), phosphorylated (p-) IRF3 (Ser396, 4947S), IRF3 (4302S), myeloid differentiation primary response 88 (MyD88, 4283S), intercellular adhesion molecule-1 (ICAM-1, 26104SF), STING (13647S) and inhibitor of κB-α (IκB-α, 4812S) were from Cell Signaling Technology. .. Antibodies against TLR4 (sc-293072), MD2 (sc-80183), and macrophage markers CD68 (sc-20060) were purchased from Santa Cruz Biotechnology.

Article Title: Cyclic‐di‐GMP induces inflammation and acute lung injury through direct binding to MD2
Article Snippet: Recombinant human MD2 (rhMD2; 1787‐MD‐050/CF) protein was obtained from R&D Systems. .. Antibodies against p38 (9212S), phosphorylated (p‐) p38 (Thr180/Tyr182, 9211S), c‐Jun N‐terminal kinase (JNK, 9252S), phosphorylated (p‐) JNK (Thr183/Tyr185, 4668S), extracellular signal‐regulated kinase (ERK, 4695S), phosphorylated (p‐) ERK (Thr202/Tyr204, 4370S), glyceraldehyde‐3‐phosphate dehydrogenase 1 (GAPDH, 5174S), TANK binding protein 1 (TBK1, 38066S), phosphorylated (p‐) TBK1(Ser172, 5483S), phosphorylated (p‐) IRF3 (Ser396, 4947S), IRF3 (4302S), myeloid differentiation primary response 88 (MyD88, 4283S), intercellular adhesion molecule‐1 (ICAM‐1, 26104SF), STING (13647S) and inhibitor of κB‐α (IκB‐α, 4812S) were from Cell Signaling Technology. .. Antibodies against TLR4 (sc‐293072), MD2 (sc‐80183), and macrophage markers CD68 (sc‐20060) were purchased from Santa Cruz Biotechnology.

Incubation:

Article Title: Enhanced NK cell activation via eEF2K-mediated potentiation of the cGAS-STING pathway in hepatocellular carcinoma.
Article Snippet: We separated 10 μg protein by 10 % SDS-PAGE, transferred the separated proteins to polyvinylidene difluoride membranes, and blocked nonspecific binding using 5 % BSA blocking buffer (Huaxingbio, HX3303). .. Subsequently, the blots were incubated overnight with the following primary antibodies at the indicated dilutions: rabbit anti-cGAS (1:500, Cell Signaling, 79978S), STING (1:500, Cell Signaling, 13647S), Phospho-STING (1:5000, Cell Signaling, 50907S), IRF3 (1:500, Abcam, ab68481), Phospho-IRF3 (1:5000, Cell Signaling, 29047S), NAK/TBK1 (1:500, Abcam, ab40676), Phospho-NAK/TBK1 (1:5000, Abcam, ab109272), and β-actin (1:5,000, Huaxingbio, HX1831) in the protein standard solution [not understood] [24]. .. Afterward, the blots were washed three times with TBST and incubated with the anti-Afterwards, we washed the blots three times with TBST and incubated them with an anti-rabbit secondary antibody (Huaxingbio, HX2030, 1:10,000) for 1 h. Finally, we visualized the blotted bands using an ECL detection system.

Article Title: Epigenetic state determines the in vivo efficacy of STING agonist therapy.
Article Snippet: Equal amounts of proteins were resolved on SDS-PAGE gels (Bio-Rad) and transferred to polyvinylidene fluoride (PVDF) membranes (Bio-Rad). .. After blocking with 5% non-fat dry milk, membranes were incubated with the following antibodies (clone, dilution, supplier, catalog no.): STING (D2P2F, 1:1000, Cell Signaling, 13647S), DNMT1 (D63A6, 1:1000, Cell Signaling, 5032S), DNMT3A (D23G1, 1:1000, Cell Signaling, 3598S), DNMT3B (D7O7O, 1:1000, Cell Signaling, 67259S), LMP2 (EPR22042, 1:1000, Abcam, ab3328), α-Tubulin (DM1A, 1:5000, Cell Signaling, 3873S), and β-Actin (AC-74, 1:5000, Sigma-Aldrich, A5316). .. Following incubation with appropriate secondary antibodies [anti-rabbit (dilution 1:2000, Cell Signaling, catalog no. 7074S), anti-mouse (dilution 1:2000,Cell Signaling, catalogno.

Article Title: AKT and EZH2 inhibitors kill TNBCs by hijacking mechanisms of involution.
Article Snippet: Pelleted cells were lysed using EBC lysis buffer (50 mM Tris, pH 8.0, 150 mM NaCl, 0.5% NP-40, 1:10,000 β mercaptoethanol, 0.5 mM EDTA) supplemented with protease inhibitors (Sigma-Aldrich, 11836153001) and phosphatase inhibitors (Sigma-Aldrich, 4906837001). .. Protein extracts were quantified using BCA reagent and then equal quantities were incubated with STING (Cell Signaling, 13647S) or IgG (Cell Signaling, 2729S) antibody and magnetic protein A/G beads (Pierce) overnight with end-over-end agitation at 4 °C. .. Protein extracts were quantified using BCA reagent and then equal quantities were incubated with STING (Cell Signaling, 13647S) or IgG (Cell Signaling, 2729S) antibody and magnetic protein A/G beads (Pierce) overnight with end-over-end agitation at 4 °C.

Blocking Assay:

Western Blot:

Article Title: Rubicon modulates neuroimmune responses following traumatic brain injury
Article Snippet: Protein signals were quantified using Image Lab software (Bio-Rad Laboratories, Inc., Hercules, CA; version 6.1.0 build 7) and normalized to loading controls. .. The following antibodies were used for immunobloting: RUBCN (CST-8465S; 68261S), NRROS (CST; 34388S), NLRP3 (CST; 15101S), cGAS (CST; 15102S), STING (CST; 13647S), α-fodrin (BML-FG6090-0100), P62 (BD Biosciences; 610832), LC3(CST; 2775S; NB100-2220), GAPDH (CST; 2118S), HMGB1(CST; 3935S), FLAG (CST; 33542S), MYC (CST; 2276S), HIS (CST; 2365S), GFP (CST; 2955S), 4-HNE (R&D Systems; MAB3249), mouse IgG1(CST; 5415S), mouse IgG2a (CST; 61656), anti-rabbit IgG-HRP (CST; 7074S), and anti-mouse IgG-HRP (CST; 7076S) Uncropped immunoblots are presented if Fig. S5 . ..

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